ABSTRACT
Non-H. pylori helicobacters (NHPH) have been demonstrated as gastric spiral-shaped bacteria in specimens obtained from dogs; however, their roles in the pathogenesis of upper gastrointestinal disease have not yet been clearly established. The purpose of this study was to evaluate the prevalence of NHPH DNA in the gastric mucosa of dogs and its association with histopathology. Helicobacter was detected through histopathological techniques, PCR, and FISH analysis from fundic biopsies of twenty dogs with or without signs of gastrointestinal disease. PCR and FISH were based on partial 16S rRNA gene sequences. Nineteen dogs showed mild to marked gastritis in the fundus, and only one dog had a healthy gastric mucosa. NHPH DNA was detected in 18 dogs with gastritis and one with normal gastric mucosa. However, there was no significant correlation between the presence of NHPH DNA and the degree of gastritis. These results show a high prevalence of NHPH DNA in the gastric mucosa of dogs from Venezuela. Further studies are necessary to determine a possible association between a specific NHPH species and the degree of gastritis.
Los helicobacteres no-H. pylori (NHPH, por sus siglas en inglés) han sido demostrados como bacterias gástricas de forma espiral; sin embargo, sus roles en la patogénesis de la enfermedad gastrointestinal superior no han sido claramente establecidos. El propósito de este estudio fue evaluar la prevalencia de ADN de los NHPH en la mucosa gástrica de perros y su asociación con histopatología. Helicobacter fue detectado a través de técnicas histopatológicas, análisis de PCR y FISH en biopsias del fundus gástrico de 20 perros con o sin signos de enfermedad gastrointestinal. La PCR y FISH se basaron en secuencias parciales del gen ARNr 16S. Diecinueve perros mostraron gastritis leve a marcada en el fundus gástrico y sólo un perro tuvo una mucosa gástrica sana. El ADN de los NHPH fue detectado en 18 perros con gastritis y uno con mucosa gástrica normal. Sin embargo, no hubo correlación significativa entre la presencia de ADN de los NHPH y el grado de gastritis. Estos resultados demuestran una alta prevalencia de ADN de los NHPH en la mucosa gástrica de perros de Venezuela. Futuros estudios son necesarios para determinar la posible asociación entre una especie específica de los HNPH y el grado de gastritis.
Subject(s)
Animals , Dogs , Female , Male , Dog Diseases/microbiology , Gastric Mucosa/microbiology , Gastritis/veterinary , Helicobacter Infections/veterinary , Helicobacter/genetics , DNA, Bacterial/analysis , Gastric Mucosa/pathology , Gastritis/microbiology , Gastritis/pathology , Helicobacter Infections/microbiology , Helicobacter/isolation & purification , In Situ Hybridization, Fluorescence , Phylogeny , Polymerase Chain Reaction/veterinary , /genetics , VenezuelaABSTRACT
Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of humans. Thirty-two bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, and 11 from intrahepatic duct stone) were obtained by percutaneous transhepatic biliary drainage. Polymerase chain reaction analysis using Helicobacter specific urease A gene and 16S rRNA primers, bile pH measurement, and Helicobacter culture were performed. Helicobacter DNA was detected in 37.5%, and 31.3% by PCR with ureA gene, and 16S rRNA, respectively. The bile pH was not related to the presence of Helicobacter. The cultures were not successful. In conclusion, Helicobacter can be detected in the bile of patients with bile duct diseases. The possibility of pathogenesis of biliary tract diseases in humans by these organisms will be further investigated.